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The EasyLife™ V is made of the following:
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The EasyLife V™ utilizes the OBB patented stroboscopic detection system – for more information on the methodology refer to technical note “EasyLife Patented Detection” (120 KB PDF). While the methodology is unique—it is well proven and tested having been pioneered by Photon Technology International Inc.™ with hundreds of systems placed and thousands of publications.
The standard system uses a specially selected PMT that allows for the measurement of lifetimes from 100 picoseconds to 3 microseconds. The wavelength range of detection is from 185 nm to 680 nm.
At the time of order you may select, as an option, an extended wavelength range PMT tube that can detect from 185 nm to 900 nm. Unfortunately nothing in life is free—the extended wavelength range will cost you more money, but in addition as dictated by physics, the near IR tube is noisier than the standard tube. Obviously still very useful, but if you do not need to measure above 680 nm, then why pay for it.
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 OBB engineers have developed a broad range of proprietary pulsed light emitting diodes (LEDs) to be used as excitation sources with the EasyLife systems. These small but robust light sources are available in a broad range of wavelengths, from UV to NIR.
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The pulsed LEDs have certain distinct advantages over more conventional light sources, such as nanosecond flash lamps and pulsed lasers, in particular that they are:
- Inexpensive
- Durable
- Require no maintenance
- Very stable pulse
- Fast
- Wavelengths from UV to NIR (280–670 nm)
- Small size
- Easily interchangeable (snap in—snap out)
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6 Hours Repeat Acquisition |
Repeat After 4 Days |
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What you need to do is to select a source or sources. The following list shows you the current choices. To exchange sources is real easy, just snap off and then snap on and it is done—no alignment required.
Below 280 nm we are limited by the current state of the technology for LED’s. Do not despair the future should offer even more choices.
| LED Sources: |
Central Wavelength |
| EL 280 |
EasyLife excitation LED: 278 nm |
| EL 295 |
EasyLife excitation LED: 295 nm |
| EL 310 |
EasyLife excitation LED: 310 nm |
| EL 340 |
EasyLife excitation LED: 340 nm |
| EL 365 |
EasyLife excitation LED: 366 nm |
| EL 370 |
EasyLife excitation LED: 375 nm |
| EL 380 |
EasyLife excitation LED: 380 nm |
| EL 393 |
EasyLife excitation LED: 395 nm |
| EL 405 |
EasyLife excitation LED: 405 nm |
| EL 410 |
EasyLife excitation LED: 410 nm |
| EL 435 |
EasyLife excitation LED: 435 nm |
| EL 445 |
EasyLife excitation LED: 445 nm |
| EL 450 |
EasyLife excitation LED: 450 nm |
| EL 460 |
EasyLife excitation LED: 463 nm |
| LD 635 |
EasyLife excitation laser diode: 630 nm |
| LD 650 |
EasyLife excitation laser diode: 650 nm |
| LD 670 |
EasyLife excitation laser diode: 670 nm |
| Other LED's and laser diodes are available on request. |
When our proprietary LED source is combined with our patented stroboscopic detection system, it offers the benefits of: high sensitivity, excellent temporal resolution and the unique ability to utilize both linear and non-linear (arithmetic and logarithmic) acquisition time base. The result is a powerful and versatile lifetime instrument, capable of handling complex multiexponential decays with an underlying broad range of lifetimes.
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Although the sample compartment is compact it has plenty of room to accommodate what you need to make a host of various sample measurements.
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10-nm bandpass interference filters are available individually. The peak transmittance wavelengths are 280, 289, 297, 308, 334 nm and from 340 nm to 650 nm at 10-nm increments. The filters should be used with the 507 filter holder (2 filter holders included with instrument). The bandpass filters are especially recommended for:
- FRET experiments when looking at donor fluorescence
- Samples which exhibit fluorescent tautomeric forms
- Excimer and exciplex systems, when looking at the monomer emission band
- Molecules, which undergo excited state proton transfer (ESPT) or intramolecular charge transfer (ICT, TICT) and exhibit dual fluorescence bands
- Experiments, which require elimination of unwanted emission at longer wavelengths
Set of eight 1" round longpass (cut-off) filters covering the wavelength range from 295–455 nm. Cut-off wavelengths are: 295, 305, 320, 380, 400, 420, 435 and 455 nm. The set is suitable for LED sources from 280 to 435 nm and fluorophores emitting at wavelengths above 300 nm.
Set of eight 1" round longpass (cut-off) filters covering the wavelength range from 475–610 nm. Cut-off wavelengths are: 475, 495, 515, 530, 550, 570, 590 and 610 nm. The set is suitable for LED sources from 280 to 580 nm and fluorophores emitting at wavelengths above 480 nm.
Set of eight 1" round longpass (cut-off) filters covering wavelength range from 630-1,000 nm. Cut-off wavelengths are: 630, 665, 695, 715, 780, 830, 850 and 1000 nm. The set is suitable for LED sources from 280 to 670 nm and fluorophores emitting at wavelengths above 650 nm.
Neutral density filter set. The neutral density filter set consists of six 1" round filters with approximate optical densities of 0.1, 0.3, 0.5, 1.0, 2.0 and 3.0 suitable for a spectral range of 300–2000 nm. The filters should be used with the 507 filter holder (2 filter holders included with instrument). The neutral density filters are used to decrease the signal intensity without changing the width of the emission slit. Keeping the slit constant is important for measuring decay curves and the corresponding IRF profile. For example, when a weakly fluorescent sample is measured with the widely open slit, a scatterer solution used to determine the IRF profile may saturate the PMT. A neutral density filter placed in the excitation or emission channel will bring the IRF intensity within the dynamic range of the detector without a need to change the emission slit width. This will ensure consistent illumination of the PMT photocathode and prevent potential targeting artifacts.
 Polarizers are used to separate the light into vertical and horizontal components. Manual sheet polarizers are an economical solution for polarization studies that have a great acceptance angle and good throughput down to approximately 310 nm.
The 'magic angle' (MA) polarizer is a sheet polarizer with the light transmission axis oriented at 54.7 deg from the vertical. It ensures that the recorded fluorescence signal is proportional to the total emission intensity and eliminates polarization artefacts caused by emission optics. In fluorescence decay measurements it removes decay distortions caused by the rotational motion of the fluorophore. The MA polarizer should be used in the emission channel in conjunction with a vertical polarizer placed in the excitation channel.
 The cold finger dewar is designed to be used with liquid nitrogen as coolant (77 K). It can also be used with organic solvent slushes at discrete temperatures above 77 K. Includes: quartz cold finger dewar that accepts 5 mm tubes, dewar holder for the single cuvette holder, foam lid for the dewar and extension collar with altered sample chamber lid, and a sample compartment. The dewar features a Suprasil quartz cold finger that passes light down to about 200 nm. Samples are placed in NMR and EPR tubes and the liquid nitrogen placed in the dewar will typically last several hours.
The front face solid sample holder insert was designed for the measurements of solid compounds or films and can be easily interchanged with the standard sample holder.
Continuously variable speed magnetic stirrer available at the time of original order. Requires a teflon-coated stirring bar (not included) suitable for a cuvette in use. Indispensable for:
- Fluorescence measurements in turbid media
- Minimizes intensity fluctuations due to photobleaching
- Eliminates intensity fluctuations due to thermal diffusion of fluorophore
- Prevents solute precipitation
The microcuvette insert supports a 3 x 3 mm 120 μl volume microcuvette with standard outer dimensions of 21 mm high by 5.4 mm wide 5.4 mm deep. The use of microcuvettes is ideal for when sample volumes are small or expensive or hard to produce such as protein work.
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