Single Channel Photometer
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 The fluorescence emission from cells on the microscope stage is measured with OBB’s proprietary 4300 photometer, featuring high sensitivity PMT detection. The 4300 photometer incorporates an eyepiece to visually observe the cells on the microscope stage. By adjusting a pair of vertical and horizontal apertures in the field of view, a small number of cells, a single cell, or even a portion of a cell can be isolated for measurements. An optional camera mount can replace the eyepiece for digital visualization. After identifying the area of interest you wish to measure, simply toggle the built-in flipping mirror from ‘view’ to ‘measure’ to direct the emission from the microscope through an interchangeable filter to the photomultiplier tube.
For dual-emission probes, refer to our dual channel photometer system or second detector channel option.
This detection system excels at applications such as:
- Fluorescence microscopy
- Quantitative FRET, FRAP, FISH
And is complementary to the following techniques:
- Live-cell imaging
- High-speed emission ratio imaging
- Electrophysiology
Key Benefits of the Single Channel Photometer:
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Inexpensive and Versatile—The OBB Photometers are universal and can adapt to any manufactures microscope via standard C-mount on the emission port. You are never captive to the manufacturer of a specific scope—hence lower costs. |
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Adjustable Area of Detection—The user can visually see and select the desired field of view for detection. |
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Eye Piece—Allows the user to visually identify the area of interest of measurement. |
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Wavelength Selection—Uses inexpensive standard one inch filters for wavelength selection. |
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Analog Detection Mode—High sensitivity and maximum dynamic range. |
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Wide Spectral Bandwidth—The ability to detect from the UV to the NIR. |
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