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 OBB Corp offers dual wavelength detection. The fluorescence emission from cell(s) on the microscope stage is measured with two independent PMT detectors mounted unto a photometer. The 4301 photometer incorporates an eyepiece to visually observe what will be detected on the microscope stage. By adjusting a pair of vertical and horizontal apertures in the field of view, a small number of cells, a single cell, or even a portion of a cell can be isolated for measurements. A diachronic beam splitter is placed in the photometer to direct the appropriate emission wavelengths to each detector for simultaneous dual detection. The diachronic beam splitter can easily be changed to select the desired emission wavelengths.
The 4301 dual photometer mounts to the C-port and is compatible with virtually all commercially available scopes. The system includes: photometer, eyepiece to view area of detection; vertical and horizontal bilateral adjustable slits to select desired area of detection; two PMT detection modules, complete with high voltage line voltage, pre-amps for side-on 1 1/8-inch diameter photomultiplier tubes; computer interface and software (Microsoft compatible); computer not included but required.
You need to select: PMT tubes (need not be the same) and diachronic cube.
This detection system excels at applications such as:
- Emission shifted dies
- Multi dies detection
- Fluorescence microscopy
- Quantitative FRET, FRAP, FISH
And is complementary to the following techniques:
- Live-cell imaging
- High-speed emission ratio imaging
- Electrophysiology
Key Benefits of the Dual Channel Photometer:
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Inexpensive and Versatile—The OBB Photometers are universal and can adapt to any manufactures microscope via standard C-mount on the emission port. You are never captive to the manufacturer of a specific scope – hence lower costs. |
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Adjustable Area of Detection—The user can visually see and select the desired field of view for detection. |
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Eye Piece—Allows the user to visually identify the area of interest of measurement. |
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Wavelength Selection—Uses inexpensive standard one inch filters for wavelength selection. |
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Analog Detection Mode—High sensitivity and maximum dynamic range. |
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Wide Spectral Bandwidth—The ability to detect from the UV to the NIR. |
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Dual Wavelength Detection—Simultaneously can detect two wavelengths. |
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Complete Software—For data collection and analysis. |
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